Application

This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.

Affects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. al. (2007) Blood. 1;110(7):2528-36).

Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).

Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).

Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8

General description

T-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Immunogen

EL-4 T lymphoma cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.

Format: Purified

Quality

Evaluated by Flow Cytometry in EL-4 T lymphoma cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.

Specificity

Clone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells.

Target description

25.24 kDa calculated.